Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation.

نویسندگان

  • Diego Romero
  • Alejandro Pérez-García
  • Jan-Willem Veening
  • Antonio de Vicente
  • Oscar P Kuipers
چکیده

A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods.

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عنوان ژورنال:
  • Journal of microbiological methods

دوره 66 3  شماره 

صفحات  -

تاریخ انتشار 2006